{ "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "# CODEX human healthy colon" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "In this tutorial we will perform a simple analysis of a published dataset from the study \"Organization of the human intestine at single-cell resolution\" https://doi.org/10.1038/s41586-023-05915-x\n", "The dataset along with all additional information can be downloaded here: https://datadryad.org/dataset/doi:10.5061/dryad.pk0p2ngrf\n", "It is already segmented, normalized and annotated, so we can directly start with spatial analysis. " ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [ "# Import SPACEc first\n", "import spacec as sp\n", "\n", "# Import other libraries that will be used\n", "import pandas as pd\n", "import time\n", "import scanpy as sc\n", "import seaborn as sns\n", "import matplotlib.pyplot as plt\n", "import seaborn as sns\n" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Read in the downloaded csv file." ] }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [ { "data": { "application/vnd.microsoft.datawrangler.viewer.v0+json": { "columns": [ { "name": "index", "rawType": "int64", "type": "integer" }, { "name": "Unnamed: 0", "rawType": "int64", "type": "integer" }, { "name": "MUC2", "rawType": "float64", "type": "float" }, { "name": "SOX9", "rawType": "float64", "type": "float" }, { "name": "MUC1", "rawType": "float64", "type": "float" }, { "name": "CD31", "rawType": "float64", "type": "float" }, { "name": "Synapto", 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| \n", " | Unnamed: 0 | \n", "MUC2 | \n", "SOX9 | \n", "MUC1 | \n", "CD31 | \n", "Synapto | \n", "CD49f | \n", "CD15 | \n", "CHGA | \n", "CDX2 | \n", "... | \n", "Cell Type em | \n", "Cell subtype | \n", "Neighborhood | \n", "Neigh_sub | \n", "Neighborhood_Ind | \n", "NeighInd_sub | \n", "Community | \n", "Major Community | \n", "Tissue Segment | \n", "Tissue Unit | \n", "
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | \n", "0 | \n", "-0.303994 | \n", "-0.163727 | \n", "-0.587608 | \n", "-0.212903 | \n", "0.164173 | \n", "-0.664863 | \n", "0.049305 | \n", "0.003616 | \n", "-0.377532 | \n", "... | \n", "NK | \n", "Immune | \n", "Mature Epithelial | \n", "Epithelial | \n", "Mature Epithelial | \n", "Epithelial | \n", "Plasma Cell Enriched | \n", "Immune | \n", "Mucosa | \n", "Mucosa | \n", "
| 1 | \n", "1 | \n", "-0.301927 | \n", "-0.491706 | \n", "-0.500804 | \n", "-0.243205 | \n", "-0.142568 | \n", "-0.664861 | \n", "-0.182627 | \n", "-0.117573 | \n", "-0.182754 | \n", "... | \n", "NK | \n", "Immune | \n", "Transit Amplifying Zone | \n", "Epithelial | \n", "Mature Epithelial | \n", "Epithelial | \n", "Mature Epithelial | \n", "Epithelial | \n", "Mucosa | \n", "Mucosa | \n", "
| 2 | \n", "2 | \n", "-0.302206 | \n", "-0.547234 | \n", "-0.510705 | \n", "-0.235309 | \n", "-0.217185 | \n", "-0.622758 | \n", "-0.296486 | \n", "-0.091504 | \n", "-0.268055 | \n", "... | \n", "NK | \n", "Immune | \n", "Innate Immune Enriched | \n", "Immune | \n", "Innate Immune Enriched | \n", "Immune | \n", "Innate Immune Enriched | \n", "Immune | \n", "Mucosa | \n", "Mucosa | \n", "
| 3 | \n", "3 | \n", "-0.304219 | \n", "-0.613068 | \n", "-0.584499 | \n", "-0.243757 | \n", "-0.266696 | \n", "-0.658449 | \n", "-0.299027 | \n", "-0.121460 | \n", "-0.345381 | \n", "... | \n", "NK | \n", "Immune | \n", "Stroma & Innate Immune | \n", "Stromal | \n", "Stroma & Innate Immune | \n", "Stromal | \n", "Stroma | \n", "Stroma | \n", "Subucosa | \n", "Submucosa | \n", "
| 4 | \n", "4 | \n", "-0.294644 | \n", "-0.615593 | \n", "-0.570580 | \n", "-0.247548 | \n", "-0.042246 | \n", "-0.642230 | \n", "-0.299031 | \n", "-0.121458 | \n", "-0.377533 | \n", "... | \n", "NK | \n", "Immune | \n", "Outer Follicle | \n", "Immune | \n", "Outer Follicle | \n", "Immune | \n", "Follicle | \n", "Immune | \n", "Mucosa | \n", "Mucosa | \n", "
| ... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "
| 2603212 | \n", "2603212 | \n", "0.351916 | \n", "0.693827 | \n", "-0.081489 | \n", "-0.240643 | \n", "0.008875 | \n", "0.143445 | \n", "0.373710 | \n", "-0.097896 | \n", "0.869830 | \n", "... | \n", "CD66+ Enterocyte | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "Secretory Epithelial | \n", "Epithelial | \n", "Mucosa | \n", "Mucosa | \n", "
| 2603213 | \n", "2603213 | \n", "0.233642 | \n", "0.171892 | \n", "0.141842 | \n", "-0.236145 | \n", "-0.097772 | \n", "-0.099283 | \n", "0.626185 | \n", "-0.105545 | \n", "0.092076 | \n", "... | \n", "CD66+ Enterocyte | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "Secretory Epithelial | \n", "Epithelial | \n", "Mucosa | \n", "Mucosa | \n", "
| 2603214 | \n", "2603214 | \n", "-0.212237 | \n", "-0.280904 | \n", "-0.197833 | \n", "-0.245638 | \n", "-0.152563 | \n", "-0.125035 | \n", "0.430416 | \n", "-0.105787 | \n", "-0.038327 | \n", "... | \n", "CD66+ Enterocyte | \n", "Epithelial | \n", "CD8+ T Enriched IEL | \n", "Immune | \n", "CD8+ T Enriched IEL | \n", "Immune | \n", "Mature Epithelial | \n", "Epithelial | \n", "Mucosa | \n", "Mucosa | \n", "
| 2603215 | \n", "2603215 | \n", "-0.328666 | \n", "0.607609 | \n", "-0.180362 | \n", "-0.247351 | \n", "-0.143742 | \n", "-0.169576 | \n", "1.095596 | \n", "-0.113879 | \n", "0.370160 | \n", "... | \n", "CD66+ Enterocyte | \n", "Epithelial | \n", "Transit Amplifying Zone | \n", "Epithelial | \n", "Mature Epithelial | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "Mucosa | \n", "Mucosa | \n", "
| 2603216 | \n", "2603216 | \n", "0.015179 | \n", "1.656635 | \n", "-0.250193 | \n", "-0.243560 | \n", "-0.084982 | \n", "0.061781 | \n", "1.300252 | \n", "-0.108526 | \n", "1.245830 | \n", "... | \n", "CD66+ Enterocyte | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "CD66+ Mature Epithelial | \n", "Epithelial | \n", "Mature Epithelial | \n", "Epithelial | \n", "Mucosa | \n", "Mucosa | \n", "
2603217 rows × 75 columns
\n", "| Cell Type | \n", "NK | \n", "Enterocyte | \n", "MUC1+ Enterocyte | \n", "TA | \n", "CD66+ Enterocyte | \n", "Paneth | \n", "Smooth muscle | \n", "Goblet | \n", "Neuroendocrine | \n", "CD57+ Enterocyte | \n", "... | \n", "B | \n", "Neutrophil | \n", "Endothelial | \n", "Cycling TA | \n", "Plasma | \n", "CD4+ T cell | \n", "Stroma | \n", "Nerve | \n", "ICC | \n", "CD7+ Immune | \n", "
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tissue_location | \n", "\n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " |
| Descending - Sigmoid | \n", "0.168867 | \n", "12.315022 | \n", "0.819044 | \n", "1.971741 | \n", "3.131373 | \n", "0.002039 | \n", "23.546279 | \n", "6.531954 | \n", "0.474376 | \n", "0.025289 | \n", "... | \n", "1.573641 | \n", "0.876556 | \n", "6.423047 | \n", "2.619063 | \n", "4.704606 | \n", "4.414188 | \n", "11.618345 | \n", "3.350818 | \n", "1.116803 | \n", "0.160709 | \n", "
| Duodenum | \n", "0.201568 | \n", "26.609101 | \n", "1.533612 | \n", "2.326641 | \n", "0.707210 | \n", "1.260529 | \n", "9.258622 | \n", "5.768660 | \n", "0.901626 | \n", "0.348572 | \n", "... | \n", "0.127139 | \n", "0.597023 | \n", "7.882344 | \n", "4.259946 | \n", "6.319595 | \n", "4.702813 | \n", "7.464375 | \n", "2.435504 | \n", "1.109816 | \n", "0.407904 | \n", "
2 rows × 25 columns
\n", "| Cell Type | \n", "NK | \n", "Enterocyte | \n", "MUC1+ Enterocyte | \n", "TA | \n", "CD66+ Enterocyte | \n", "Paneth | \n", "Smooth muscle | \n", "Goblet | \n", "Neuroendocrine | \n", "CD57+ Enterocyte | \n", "... | \n", "B | \n", "Neutrophil | \n", "Endothelial | \n", "Cycling TA | \n", "Plasma | \n", "CD4+ T cell | \n", "Stroma | \n", "Nerve | \n", "ICC | \n", "CD7+ Immune | \n", "
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Major Community | \n", "\n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " | \n", " |
| Epithelial | \n", "0.161610 | \n", "37.679507 | \n", "1.355490 | \n", "4.756104 | \n", "4.115486 | \n", "0.471243 | \n", "2.195573 | \n", "12.908940 | \n", "1.269589 | \n", "0.261586 | \n", "... | \n", "0.045134 | \n", "0.324677 | \n", "3.203576 | \n", "8.585738 | \n", "2.040757 | \n", "2.644976 | \n", "2.636241 | \n", "1.077888 | \n", "0.667796 | \n", "0.638192 | \n", "
| Immune | \n", "0.280478 | \n", "20.737929 | \n", "1.939399 | \n", "1.456782 | \n", "0.653502 | \n", "1.486818 | \n", "7.420291 | \n", "4.415806 | \n", "0.780955 | \n", "0.336899 | \n", "... | \n", "1.705599 | \n", "0.866600 | \n", "8.316928 | \n", "1.787186 | \n", "12.491628 | \n", "8.588882 | \n", "5.208126 | \n", "2.183752 | \n", "1.333793 | \n", "0.236641 | \n", "
| Smooth Muscle | \n", "0.058673 | \n", "0.638322 | \n", "0.058673 | \n", "0.184112 | \n", "0.055638 | \n", "0.036418 | \n", "61.333495 | \n", "0.237727 | \n", "0.024278 | \n", "0.003035 | \n", "... | \n", "0.011128 | \n", "0.443082 | \n", "4.899194 | \n", "0.349003 | \n", "0.144659 | \n", "0.678786 | \n", "12.796779 | \n", "7.355366 | \n", "0.903362 | \n", "0.011128 | \n", "
| Stroma | \n", "0.130188 | \n", "1.807237 | \n", "0.236579 | \n", "0.065794 | \n", "0.282775 | \n", "0.131588 | \n", "12.944635 | \n", "0.111990 | \n", "0.004200 | \n", "0.008399 | \n", "... | \n", "0.044796 | \n", "1.625254 | \n", "18.999090 | \n", "0.090992 | \n", "0.377966 | \n", "1.814237 | \n", "36.049556 | \n", "3.552880 | \n", "1.922027 | \n", "0.034997 | \n", "
4 rows × 25 columns
\n", "| \n", " | x | \n", "y | \n", "Cell Type | \n", "unique_region | \n", "
|---|---|---|---|---|
| 2192275 | \n", "583.0 | \n", "8076.0 | \n", "Cycling TA | \n", "B012_Sigmoid | \n", "
| 2192276 | \n", "1092.0 | \n", "3971.0 | \n", "Cycling TA | \n", "B012_Sigmoid | \n", "
| 2192277 | \n", "830.0 | \n", "5034.0 | \n", "Cycling TA | \n", "B012_Sigmoid | \n", "
| 2192278 | \n", "1278.0 | \n", "7631.0 | \n", "Cycling TA | \n", "B012_Sigmoid | \n", "
| 2192279 | \n", "628.0 | \n", "2757.0 | \n", "Cycling TA | \n", "B012_Sigmoid | \n", "
| ... | \n", "... | \n", "... | \n", "... | \n", "... | \n", "
| 2329692 | \n", "7825.0 | \n", "7398.0 | \n", "DC | \n", "B012_Sigmoid | \n", "
| 2329693 | \n", "4960.0 | \n", "5855.0 | \n", "DC | \n", "B012_Sigmoid | \n", "
| 2329694 | \n", "6684.0 | \n", "2653.0 | \n", "DC | \n", "B012_Sigmoid | \n", "
| 2329695 | \n", "5280.0 | \n", "8654.0 | \n", "DC | \n", "B012_Sigmoid | \n", "
| 2329696 | \n", "8860.0 | \n", "8778.0 | \n", "DC | \n", "B012_Sigmoid | \n", "
51593 rows × 4 columns
\n", "